Biological wastewater treatment (anaerobic and aerobic digestion reactors) takes advantage of the ability of certain microorganisms (including bacteria) to assimilate organic matter and nutrients dissolved in the water for their own growth, thus removing soluble components in the water. Soluble organic matter is assimilated by microorganisms as a carbon source. After this operation, the biomass produced from the supernatant is then separated by decantation. For microorganisms to grow, nitrogen and phosphorus are needed in the effluent as well as organic matter. If their concentration is not sufficient, they have to be added to the treatment.

The traditional application consists of removing biodegradable organic matter, both soluble and colloidal, and compounds containing nitrogen and phosphorus. It is one of the most common treatments for urban wastewater and most industrial water, due to its simplicity and low operating economic cost.

The only requirements for the successful application of these technologies are that the contamination is biodegradable and that there are no biocidal compounds in the effluent to be treated.

Microorganisms can assimilate organic matter either with or without the presence of oxygen; thus, there are two main biological treatment systems for wastewater:

The most suitable type of biological process is chosen after analyzing the features of the effluent to be treated on a case by case basis.


Aerobic wastewater treatment systems take advantage of the ability of microorganisms to grow by assimilating organic matter and nutrients (nitrogen and phosphorus) dissolved in the water in the presence of oxygen, which acts as an electron acceptor when oxidizing the organic matter.

This phenomenon leads to high energy yields and significant production of sludge, resulting from the high growth of bacteria under aerobic conditions.

Aerobic treatment stages


An aerobic treatment system consists of the following stages:

1. Pre-treatment

Large size solids arriving at the water treatment plant are first removed. If not disposed of effectively, these materials can lead to serious equipment failure. Stones, sand, tin cans, etc. cause significant wear on pipes as well as pumps.

Oils and fats are also removed at this stage to prevent the biological treatment from slowing down, as both the performance and the quality of the effluent decreases. Both physical and mechanical operations are used for this. The main operations used depend on the wastewater source, its quality and subsequent treatment:

  • Separation of large solids: This system is used whenever the water to be treated may contain large solids. It consists of a pit at the collector entrance to concentrate the solids and decant the sand to a specific area where it can be extracted effectively.
  • Gross filter: This operation prevents the massive arrival of large solids from obstructing later parts of the process. It consists of bars with different distances between them to separate the solids according to their size.
  • Screening: This operation is required when wastewater contains large amounts of floating solids or debris. Sieves of different thickness are used.
  • Desander: For removing solid particles larger than 200 microns which can block pipes or pumps and can scratch and damage different pieces of equipment.
  • Degreasing: To remove grease, oils, foam and other floating materials lighter than water, which can affect subsequent treatment processes. This is normally done by blowing in air to demulsify and make the grease float.

2. Primary treatment

At this stage, suspended solids are removed from the water using different physical and chemical processes. These solids can be sedimentable, floating or colloidal.

  • Sedimentation: Separation under gravity results in particles denser than water being deposited at the bottom of the settler. The greater the size and density of the particles to be separated in the water, the higher the sedimentation velocity, and this is the main design parameter for this equipment. This sedimentation operation is also known as decantation. Primary decantation removes suspended solids (60% approx.) and organic matter (30% approx.) and protects subsequent biological oxidation processes from the intrusion of inert, high density sludge. There are rectangular, circular and lamellar settlers that can be used.
  • Flotation: This is based on a difference in densities and separates solid or liquid matter which is less dense than the fluid, where it rises to the surface. Air is used as a flotation agent and, depending on how it is introduced into the liquid, there are two flotation systems: Dissolved Air Flotation (DAF), which introduces air into the wastewater under a pressure of several atmospheres; and Induced Gas Flotation, where gas bubbles are produced via diffusers.
  • Coagulation – Flocculation: If very small particles are present, highly stable colloidal suspensions are formed, due to the electrical interactions between them, and their sedimentation rate is slow. Thus, to improve their removal, chemical reagents are added to destabilize the colloidal suspension (coagulation) and promote their flocculation to obtain easily settleable particles. Coagulants are usually chemicals with an opposite electric charge in solution to the colloid.
  • Filtration: The water is filtered through a porous medium to retain as much of the suspended material as possible. The porous medium traditionally used is a bed of sand of varying height.

3. Secondary treatment

This is based on biological processes which use microorganisms (especially bacteria) to remove biodegradable organic matter, both colloidal and dissolved, and nutrients (compounds containing N and P). In most cases, organic matter is oxidized by the microorganisms which use it as a source of energy for growth.

Aerobic processes are based on the elimination of organic pollutants by their transformation into bacterial biomass with the help of oxygen (which acts as an electron acceptor in the oxidation process), CO2 and H2O.

  • Digestion: In aerobic systems, wastewater passes through an aerobic reactor-digester containing the microorganisms which oxidize dissolved organic matter via an oxygen flow.
  • Decantation: The sludge formed is separated by gravity in secondary settlers.

4. Tertiary treatment

Depending on the quality of the effluent obtained, its final destination and relevant legislation, tertiary treatment may be applied to remove residual organic load and other pollutants not removed after the secondary treatment, such as nutrients, phosphorus and nitrogen. Any combination of treatment processes can be used, whether physical, chemical or biological. Generally, they are:

  • Filtration: Microfiltration, ultrafiltration.
  • Ionic exchange.
  • Reverse osmosis.
  • Adsorption.
  • Membranes.
  • Disinfection. This consists of removing or inactivating pathogenic or any other living microorganisms to ensure the treated water can be reused. The main disinfection processes are:
    • Chlorination
    • Ozonation
    • Electrodisinfection


Depending on the system used for the growth of biomass, aerobic treatment systems are classified as follows:

1. Biomass in suspension (activated sludge). Biomass grows freely or in suspension inside the bioreactor and produces flocs.

  • Conventional process
  • Sequential Batch Reactors (SBR): This type of treatment is in batch form with all processes occurring in the same tank sequentially over time. It is a good alternative for those industries that have small effluents but with highly variable properties.Sequencial bath reactors SBR
  • Membrane Bioreactor (MBR): This is similar to the activated sludge method but with an ultrafiltration membrane module inside to separate the sludge and the liquid via membranes. This has significant advantages over traditional secondary decanters and is an alternative where there is little space.Membrane bioreactor MBR
  • BIOCARB®: This is an exclusive process patented by Condorchem Envitech and is based on a fixed bed aerobic reactor with granular activated carbon as filler material. The carbon filters, adsorbs and acts as a support for the biofilm, in addition to feeding the microorganisms with minerals and trace elements. The adsorption process provides a double contribution to the process, by laminating the pollutant loading peaks and by increasing the residence time of the contaminants inside the reactor so the persistent organic compounds can break down. The BioCarb® reactor has proven especially effective in treating colored contaminants difficult to biodegrade. In addition, the immobilization of the biomass on the surface of the lignite carbon means the wastewater is biologically and physicochemically treated in a single stage.

aerobic reactors biocarb


2. Fixed biomass. The biomass grows attached to a natural or artificial support where it forms a layer or film.

  • Biodiscs: This consists of a set of discs of a certain material (e.g. wood, corrugated polyethylene or polystyrene and PVC) that revolve around a horizontal axis, located inside the reactor. A bacterial biomass film gradually forms on this medium, and uses the soluble organic matter in the wastewater as a substrate for its metabolism. When the surface of the disc is in contact with the air, the biomass attached to the disc takes the oxygen necessary for the degradation of the organic matter during the immersion period.Biodiscs
  • Biofilters: The air is pulled near the source of emanation and led into a conditioning chamber, where it is saturated with moisture and directed to a fixed biomass bed. Contaminants are absorbed onto the biomass biofilm which forms on the filler and are subsequently digested by microorganisms. These digestion and metabolization processes convert the compounds into ones that no longer smell.
  • Trickling filters: Wastewater flows or is sprayed onto the filter. As it passes through the filter pores, the organic matter is degraded by the biomass covering the filter material.Tricklinkg filters
  • Moving bed biofilm reactor (MBBR): The purifying bacterial culture is in the form of a biofilm adhered to specific large surface supports (filter fill) which are submerged and moving in the biological reactor.

Moving bed biofilm reactor MBR

Selecting the most convenient biomass in suspension or fixed biomass biological process is done after analyzing the effluent properties, type of industrial process producing it, the degree of purification required and the global needs of the user:

fixed biomass vs biomass in suspension


The criteria used to select whether an aerobic process is more appropriate or if an anaerobic process is preferable are:

  • Concentration of organic matter to be removed.
  • If nitrogen removal is necessary.
  • Availability of physical space.
  • Project OPEX and CAPEX costs.

The following table shows which kind of process (aerobic or anaerobic) is preferred according to these criteria:

aerobic systems vs anaerobic treatment

Advantages of Aerobic systems:

  • A wide variety of wastewater can be treated: the two requirements are they must be biodegradable.
  • Higher yield than anaerobic = 0.4 (1g of organic matter for 0.4g of biomass).
  • Ease of operation.
  • Low CAPEX.
  • Minimizes production of odors.
  • Reduces coliforms, pathogens and fats.
  • Produces clarified supernatant.
  • A greater number of bacteria types can be used for digestion.
  • Lesser sludge respiration rate.

Disadvantages of Aerobic systems:

  • High OPEX due to continuous energy expenditure associated with aeration.
  • Many parameters to control for optimum results: pH, Temperature, % Organic matter, Inlet flow, % toxic compounds (biocides).
  • Higher costs during maintenance or breakdown, due to having to maintain the organic matter levels necessary for the microorganisms to survive.


One of the biggest challenges of aerobic systems is the management of the sludge produced. The following diagram shows the general sludge management process, depending on the amount.

Currently, anaerobic treatment is used for sludge management after aerobic treatment in many cases and whenever possible.

sludge treatment